Product Name: Furantoin Elisa Kit Trademark: Safetix Specification: 96test/kit Origin: Hangzhou HS Code: 3822001000 Product Description The Furantoin Elisa Kit is an indirect method competitive enzyme-linked immunoassay forthe quantitative detects the presence of Furantoin residue in samples of aquatic products,tissues (pork, beef, lamb, chicken, etc.), and egg.Incubation Time: 30min + 15minAssay Time Requirement: Less than 2 hoursDetection Range: 0.05-4.05ppbPRINCIPLE The Furantoin Elisa Kit is an indirect method competitive enzyme-linked immunoassay. Theassay is performed in ELISA microwells, which are pre-coated with Furantoin antigen.When sample is placed into a microwell, the free Furantoin antigens in the sample willcompete with the pre-coated Furantoin antigens to combine the Furantoin antibodies. TheFurantoin-antibodies complex will bind to the enzyme conjugated secondary antibody. After that, the unbound materials will be removed in a washing step. Then, two substratesare added into the microwell. The bound enzyme conjugate will convert the colorlesssubstrate into blue. The color will change from blue to yellow after adding stop solution.Absorbance is then measured spectrophotometrically (450 nm) and colour intensity resultsare inversely proportional to the original Furantoin content in the sample. Sampleconcentrations are then calculated on the basis of a calibration curve derived from thecalibrators.